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The DNA of bacteriphage 643: Isolation and properties of the DNA of a bacteriphage infecting lactic streptococci

Identifieur interne : 004E28 ( Main/Exploration ); précédent : 004E27; suivant : 004E29

The DNA of bacteriphage 643: Isolation and properties of the DNA of a bacteriphage infecting lactic streptococci

Auteurs : David J. Lyttle [Nouvelle-Zélande] ; George B. Petersen [Nouvelle-Zélande]

Source :

Virology [ 0042-6822 ] ; 1984.

RBID : ISTEX:23FA45771099CB47EA4326F4F7952BE92E0514E5

English descriptors

Teeft :
- Academic press, Agarose, Alkaline sucrose gradients, Analytical ultracentrifuge, Bacteriophage, Band pattern, Band sedimentation, Band sedimentation velocity, Base composition, Base pairs, Biol, Buoyant density, Cauliflower, Cauliflower mosaic virus, Cell biology, Circular dimeric form, Circular molecule, Circular monomeric form, Coli polymerase, Contour length, Contour lengths, Cscl, Denatured, Denatured bacteriophage, Denatured phage, Denaturing solvents, Deoxyribonucleic acid, Digestion, Electron micrographs, Electron microscopy, Electrophoresis, Electrophoretic mobilities, Endonuclease, Equal volume, Fragment, Genome, Hinfi, Klenow, Klenow polymerase, Large products, Linear forms, Linear molecules, Liquid scintillation, Lyttle, Major species, Maximum fraction, Molecular sizes, Molecular weight, Molecular weight marker, Molecular weights, Molecule, Monomeric, Nacl, Native bacteriophage, Native phage, Neutral sucrose gradients, Nick translation, Nuclease, Petersen, Phage, Phage lambda, Phage particles, Polymerase, Predominant species, Restriction endonuclease, Room temperature, Sedimentation, Sedimentation coefficient, Sedimentation coefficients, Sedimentation studies, Sedimentation velocity, Singlestranded region, Specific nuclease, Spinco rotor, Streptococcus cremoris, Sucrose, Sucrose gradients, Zone sedimentation.

Abstract

Abstract: The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 × 106. The four species observed on electrophoresis are believed to be a circular monomeric form, a linear form derived from it, a circular dimeric form, and the corresponding linear dimer. Two DNA components were demonstrated by sedimentation velocity in the analytical ultracentrifuge and by zone sedimentation in sucrose gradients. These corresponded to a circular monomeric form and a circular dimeric form; no evidence for supercoiled forms was found by sedimentation in either neutral or denaturing solvents. Bacteriophage 643 DNA was sensitive to cleavage by single-strand-specific nuclease S1 and could be labeled in vitro by the PolI-catalyzed incorporation of [α-32P]dATP into the molecule under conditions that did not permit nick translation, suggesting that the circular duplex molecule is interrupted by a single-stranded gap.

Url:

https://api.istex.fr/ark:/67375/6H6-TRNF7M09-F/fulltext.pdf

DOI: 10.1016/0042-6822(84)90406-9

Affiliations:

Nouvelle-Zélande

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 × 106. The four species observed on electrophoresis are believed to be a circular monomeric form, a linear form derived from it, a circular dimeric form, and the corresponding linear dimer. Two DNA components were demonstrated by sedimentation velocity in the analytical ultracentrifuge and by zone sedimentation in sucrose gradients. These corresponded to a circular monomeric form and a circular dimeric form; no evidence for supercoiled forms was found by sedimentation in either neutral or denaturing solvents. Bacteriophage 643 DNA was sensitive to cleavage by single-strand-specific nuclease S1 and could be labeled in vitro by the PolI-catalyzed incorporation of [α-32P]dATP into the molecule under conditions that did not permit nick translation, suggesting that the circular duplex molecule is interrupted by a single-stranded gap.</div>
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The DNA of bacteriphage 643: Isolation and properties of the DNA of a bacteriphage infecting lactic streptococci

The DNA of bacteriphage 643: Isolation and properties of the DNA of a bacteriphage infecting lactic streptococci

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri